Introduction of subwavelength inclusions in the core opens up an additional degree of freedom in shaping of dispersion characteristic in photonic crystal fibers (PCFs). We have developed a PCF with a nanostructured inclusion in the core to verify this concept. To suppress higher order modes, the photonic cladding structure of the developed fiber is composed of a first ring with linear filling factor of 0.95 and the remaining 5 rings with a lower linear filling factor of 0.4 with a lattice constant of 2.6μm. Diameter of the nano-inclusion in the core is 410 nm. For the fiber development, we used a pair of thermally matched soft glasses: Schott SF6 lead glass and an in-house synthesized NC21 borosilicate glass. In this paper, we report on dispersion measurements using a spectral interferomeric technique. A dispersion unbalanced Mach–Zehnder interferometer, combined with a supercontinuum source is used. Dispersion characteristics in wide range of wavelengths extending from 0.65 to 1.6μm, are measured and verified against calculated results.